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Team:Freiburg
From 2013.igem.org
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Revision as of 21:43, 16 October 2013
Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene
regulation in mammalian cells. Therefore, we engineered the recently understood and highly promising
CRISPR/Cas9 system.
The regulation is based on the RNA-guided dCas9 protein, which allows targeting of specific DNA sequences. Our toolkit comprises not only a standardized dCas9 protein, but also different effector domains for efficient gene activation or repression. 
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