Team:Evry/Notebook/w2

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Iron coli project

Week 2: 24th June - 30th June

Monday, June 24th

Competents cells made on friday 21st was plated and incubated the whole week-end at 30°C. Today we analyzed results and it seems that medium had been contaminated.

New competents cells will be made.

Tuesday, June 25th

New competent cells have been made with the same strains of bacteria. We thought that the contamination of our cells was due either to the solution of CaCl2 that we used which may had not been sterile and/or incorrect manipulations. This time, we prepared new CaCl2 solutions that we made sure to autoclave correctly. Then, same tests as we did before of contamination and competence have been made.

Wednesday, June 26th

The DH5a strain was not contaminated and competent. The TOP10 strain was contaminated only on the Kanamycin petri dish and competent. The BL21 strain was contaminated and not usable.

Thurdsay, June 27th

We designed the plasmid constructions and primers we will use.

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