Team:Freiburg/HumanPractice/experts

From 2013.igem.org

(Difference between revisions)
Line 88: Line 88:
<table class="imgtxt" style="width:900px;">
<table class="imgtxt" style="width:900px;">
<tr>
<tr>
-
<td><p id="interview_name"><center> Prof. Dr. Peter Stäheli </center></p></td>
+
<td><p id="interview_name"><center> Dr. Satoru Watanabe  </center></p></td>
<td rowspan="3">
<td rowspan="3">
<p>
<p>
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“… in this project there are no viruses or stable insertions involved. So biosafety should be of less concern …”
</p>
</p>
<p style="padding-left: 30px; padding-top:30px;">
<p style="padding-left: 30px; padding-top:30px;">
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text v text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“… in combination with a riboswitch, gene regulation by dCas9 might be controlled more strictly by controlling dCas9 expression …”
</p>
</p>
</td>
</td>
Line 102: Line 102:
</tr>
</tr>
<tr>
<tr>
-
<td><p id="interview_institut"><center>Virology </center></p></td>
+
<td><p id="interview_institut"><center>Genetics of Cyanobacteria</center></p></td>
</tr>
</tr>
Line 115: Line 115:
<td rowspan="3">
<td rowspan="3">
<p>
<p>
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“… an analogously designed uniCAS plant repressor system could one day provide an efficient alternative to high-throughput RNAi screens ...“
</p>
</p>
<p style="padding-left: 30px; padding-top:30px;">
<p style="padding-left: 30px; padding-top:30px;">
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text v text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“... what about directing dCas9-GFP to repetitive and adjacent loci on a chromsome - whereby gaining a new variant of Fluorescence in-situ hybridization?“
</p>
</p>
</td>
</td>
-
<td><p id="interview_name"><center> Prof. Dr. Peter Stäheli </center></p></td>
+
<td><p id="interview_name"><center> Prof. Dr. Andreas Hiltbrunner  </center></p></td>
</tr>
</tr>
<tr>  
<tr>  
Line 127: Line 127:
</tr>
</tr>
<tr>
<tr>
-
<td><p id="interview_institut"><center>Virology </center></p></td>
+
<td><p id="interview_institut"><center>Light perception of plants</center></p></td>
</tr>
</tr>
Line 139: Line 139:
<table class="imgtxt" style="width:900px;">
<table class="imgtxt" style="width:900px;">
<tr>
<tr>
-
<td><p id="interview_name"><center> Prof. Dr. Peter Stäheli </center></p></td>
+
<td><p id="interview_name"><center> Dr. Jochen Holzschuh  </center></p></td>
<td rowspan="3">
<td rowspan="3">
<p>
<p>
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“... I would use this kit to understand the
 +
transcriptional codes of brain development better, by
 +
simultaneously regulating various promoters of neural
 +
transcription factors ...“
</p>
</p>
<p style="padding-left: 30px; padding-top:30px;">
<p style="padding-left: 30px; padding-top:30px;">
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text v text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
„... as a scientist who creates a certain knowledge,
 +
responsibility does not stop at the end of his or her
 +
own work - instead, the duty of observing the
 +
usage of this knowledge by other scientists arises ...“
</p>
</p>
</td>
</td>
Line 153: Line 159:
</tr>
</tr>
<tr>
<tr>
-
<td><p id="interview_institut"><center>Virology </center></p></td>
+
<td><p id="interview_institut"><center>Neuronal Development</center></p></td>
</tr>
</tr>
Line 166: Line 172:
<td rowspan="3">
<td rowspan="3">
<p>
<p>
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
„... Cas9, but only when catalytically functional, may become crucially important for improved herbicide resistance of crops. Regulation alone will probably be insufficient for this issue ...“
</p>
</p>
<p style="padding-left: 30px; padding-top:30px;">
<p style="padding-left: 30px; padding-top:30px;">
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text v text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“… for CRISPR/Cas9 use in plants, stable integration of the components would be necessary – in combination with tissue specific promoters, spatial constraints may be circumvented …”
</p>
</p>
</td>
</td>
-
<td><p id="interview_name"><center> Prof. Dr. Peter Stäheli </center></p></td>
+
<td><p id="interview_name"><center> Prof. Dr. Peter Beyer </center></p></td>
</tr>
</tr>
<tr>  
<tr>  
Line 178: Line 184:
</tr>
</tr>
<tr>
<tr>
-
<td><p id="interview_institut"><center>Virology </center></p></td>
+
<td><p id="interview_institut"><center>Cell biology of plants</center></p></td>
</tr>
</tr>
Line 190: Line 196:
<table class="imgtxt" style="width:900px;">
<table class="imgtxt" style="width:900px;">
<tr>
<tr>
-
<td><p id="interview_name"><center> Prof. Dr. Peter Stäheli </center></p></td>
+
<td><p id="interview_name"><center> Dr. Georgios Pyrowolakis  </center></p></td>
<td rowspan="3">
<td rowspan="3">
<p>
<p>
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“... behavioral genetics of Drosophila melanogaster can be efficiently enhanced through tools such as the CRISPR/Cas9 and uniCAS systems ...“
</p>
</p>
<p style="padding-left: 30px; padding-top:30px;">
<p style="padding-left: 30px; padding-top:30px;">
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text v text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“… a maybe possible stable control of complex behavior will raise the question if we are allowed to steer animals …”
</p>
</p>
</td>
</td>
Line 204: Line 210:
</tr>
</tr>
<tr>
<tr>
-
<td><p id="interview_institut"><center>Virology </center></p></td>
+
<td><p id="interview_institut"><center>Fly Development</center></p></td>
</tr>
</tr>
Line 217: Line 223:
<td rowspan="3">
<td rowspan="3">
<p>
<p>
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“… genome engineering by CRISPR/Cas9 is a breakthrough that only happens every 10 to 20 years …”
</p>
</p>
<p style="padding-left: 30px; padding-top:30px;">
<p style="padding-left: 30px; padding-top:30px;">
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text v text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“… the great advantage of repression with dCas9 - in comparison to a complete gene knock out - is, that even vital genes can be targeted …”
</p>
</p>
</td>
</td>
-
<td><p id="interview_name"><center> Prof. Dr. Peter Stäheli </center></p></td>
+
<td><p id="interview_name"><center> Prof. Dr. Wolfgang Hess </center></p></td>
</tr>
</tr>
<tr>  
<tr>  
Line 229: Line 235:
</tr>
</tr>
<tr>
<tr>
-
<td><p id="interview_institut"><center>Virology </center></p></td>
+
<td><p id="interview_institut"><center>Genetics of Cyanobacteria</center></p></td>
</tr>
</tr>
Line 241: Line 247:
<table class="imgtxt" style="width:900px;">
<table class="imgtxt" style="width:900px;">
<tr>
<tr>
-
<td><p id="interview_name"><center> Prof. Dr. Peter Stäheli </center></p></td>
+
<td><p id="interview_name"><center> Dr. Ekkehard Schulze  </center></p></td>
<td rowspan="3">
<td rowspan="3">
<p>
<p>
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“… the ageing processes, where hundreds of genes are involved, could be further investigated …”
</p>
</p>
<p style="padding-left: 30px; padding-top:30px;">
<p style="padding-left: 30px; padding-top:30px;">
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text v text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
“… the required dCas9 and crRNA vectors can be easily stably introduced into C. elegans  …”
</p>
</p>
</td>
</td>
Line 255: Line 261:
</tr>
</tr>
<tr>
<tr>
-
<td><p id="interview_institut"><center>Virology </center></p></td>
+
<td><p id="interview_institut"><center>Genetics of nematodes</center></p></td>
</tr>
</tr>
Line 268: Line 274:
<td rowspan="3">
<td rowspan="3">
<p>
<p>
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
"... the attractiveness of this system emerges from the possibility of regulating whole clusters of interacting genes ...“
</p>
</p>
<p style="padding-left: 30px; padding-top:30px;">
<p style="padding-left: 30px; padding-top:30px;">
-
text text text text text text text text text text text text text text text text text text text text text text text text text text text text v text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text text
+
"... in terms of ethical discussions, this new method is not more concerning than ordinary knock-out techniques ...“
</p>
</p>
</td>
</td>
-
<td><p id="interview_name"><center> Prof. Dr. Peter Stäheli </center></p></td>
+
<td><p id="interview_name"><center> Prof. Dr. Annette Neubüser  </center></p></td>
</tr>
</tr>
<tr>  
<tr>  
Line 280: Line 286:
</tr>
</tr>
<tr>
<tr>
-
<td><p id="interview_institut"><center>Virology </center></p></td>
+
<td><p id="interview_institut"><center>Developmental Biology</center></p></td>
</tr>
</tr>

Revision as of 18:12, 27 October 2013

Expert Opinions

Expert Interviews

We wanted to know if experts on the field of biology and medicine are interested in using our uniCAS toolkit. We were curious to learn about their application ideas and also wanted to know their thoughts about safety and ethics.

.

Prof. Dr. Wolfgang Driever

“… together with TALEs, CRISPR/Cas offers a cheap tool to control gene expression without the need of creating transgenic organisms - which is certainly also a benefit from an ethical point of view …”

“… promoter activities are complexly regulated. Thus, we often need more than one copy of a transcriptional regulation domain, such as VP16, targeted to the same gene of interest …”

Developmental Biology

“… tools such as dCas9 or TAL effectors definitely provide a first glance onto the medicine of tomorrow …”

“... eukaryotic cells are way too complex. Even an efficient regulation of 10 genes at once is only a drop in the bucket …”

Prof. Dr. Peter Stäheli

Virology

Dr. Satoru Watanabe

“… in this project there are no viruses or stable insertions involved. So biosafety should be of less concern …”

“… in combination with a riboswitch, gene regulation by dCas9 might be controlled more strictly by controlling dCas9 expression …”

Genetics of Cyanobacteria

“… an analogously designed uniCAS plant repressor system could one day provide an efficient alternative to high-throughput RNAi screens ...“

“... what about directing dCas9-GFP to repetitive and adjacent loci on a chromsome - whereby gaining a new variant of Fluorescence in-situ hybridization?“

Prof. Dr. Andreas Hiltbrunner

Light perception of plants

Dr. Jochen Holzschuh

“... I would use this kit to understand the transcriptional codes of brain development better, by simultaneously regulating various promoters of neural transcription factors ...“

„... as a scientist who creates a certain knowledge, responsibility does not stop at the end of his or her own work - instead, the duty of observing the usage of this knowledge by other scientists arises ...“

Neuronal Development

„... Cas9, but only when catalytically functional, may become crucially important for improved herbicide resistance of crops. Regulation alone will probably be insufficient for this issue ...“

“… for CRISPR/Cas9 use in plants, stable integration of the components would be necessary – in combination with tissue specific promoters, spatial constraints may be circumvented …”

Prof. Dr. Peter Beyer

Cell biology of plants

Dr. Georgios Pyrowolakis

“... behavioral genetics of Drosophila melanogaster can be efficiently enhanced through tools such as the CRISPR/Cas9 and uniCAS systems ...“

“… a maybe possible stable control of complex behavior will raise the question if we are allowed to steer animals …”

Fly Development

“… genome engineering by CRISPR/Cas9 is a breakthrough that only happens every 10 to 20 years …”

“… the great advantage of repression with dCas9 - in comparison to a complete gene knock out - is, that even vital genes can be targeted …”

Prof. Dr. Wolfgang Hess

Genetics of Cyanobacteria

Dr. Ekkehard Schulze

“… the ageing processes, where hundreds of genes are involved, could be further investigated …”

“… the required dCas9 and crRNA vectors can be easily stably introduced into C. elegans …”

Genetics of nematodes

"... the attractiveness of this system emerges from the possibility of regulating whole clusters of interacting genes ...“

"... in terms of ethical discussions, this new method is not more concerning than ordinary knock-out techniques ...“

Prof. Dr. Annette Neubüser

Developmental Biology

Retrieved from "http://2013.igem.org/Team:Freiburg/HumanPractice/experts"