Team:UFMG Brazil/lab/results
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We have constructed the composite RCNA+YFP to detect IMA (ischemia modified albumin) in the serum of patients with cardiac risk. These patients present more IMA than normal individuals. | We have constructed the composite RCNA+YFP to detect IMA (ischemia modified albumin) in the serum of patients with cardiac risk. These patients present more IMA than normal individuals. | ||
- | To prove that our construct works, we made some tests with transformed ''E. coli'' XL1-Blue. We added different concentrations of cobaltous chloride to bacterial cultures and measured their fluorescence (excitation: 514nm; emission: 527 nm) and absorbance (600 nm) for a certain period. The results (Figures 6 to 8) show a peak after | + | To prove that our construct works, we made some tests with transformed ''E. coli'' XL1-Blue. We added different concentrations of cobaltous chloride to bacterial cultures and measured their fluorescence (excitation: 514nm; emission: 527 nm) and absorbance (600 nm) for a certain period. The results (Figures 6 to 8) show a peak of fluorescence which can be seen 1 hour after 25 µM treatment, and another peak after 4 hours of 75µM treatment. |
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+ | [[File:ima_fluo_barra.jpg|700px|thumb|center|'''Figure 6: Fluorimetric reads of cultures ''of E. coli'' XL1-Blue carrying the plasmid PSB1A3_RCNA+ YFP, after treatment with different concentrations of cobalt.''' Bacteria were treated with 0, 25, 50, 75, 100, 125 or 150 µM of cobalt chloride. After that, fluorescence was read hourly, until 4 hours, and then it was read 8 and 24 hours after treatment. A peak of fluorescence can be seen 1 hour after 25 µM treatment, and another peak after 4 hours of 75µM treatment.]] | ||
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Revision as of 20:11, 27 October 2013