Team:UFMG Brazil/lab/results
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== Discussion and Conclusions == | == Discussion and Conclusions == | ||
- | The peak of fluorescence after 3 hours (Figures 6 to 8) is probably related to the phase of growth in which bacteria are (exponential phase). At this phase, bacteria are more metabolically active, once they are dividing in a great rate, so they need to produce large amounts of proteins. | + | <!--The peak of fluorescence after 3 hours (Figures 6 to 8) is probably related to the phase of growth in which bacteria are (exponential phase). At this phase, bacteria are more metabolically active, once they are dividing in a great rate, so they need to produce large amounts of proteins. |
Concerning cobalt concentrations, it is likely that lower concentrations activate the promoter less than intermediate concentrations, whereas bigger concentrations might be saturating the promoter, or even causing negative feedback. | Concerning cobalt concentrations, it is likely that lower concentrations activate the promoter less than intermediate concentrations, whereas bigger concentrations might be saturating the promoter, or even causing negative feedback. | ||
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Our results show that the composite RCNA+YFP generates fluorescence in the presence of cobalt. Furthermore, it can be used to distinguish between ischemic and non ischemic individuals. Further characterization, including usage of samples containing human IMA (ischemia modified albumin) and normal albumin, is needed, in order to improve our composite’s documentation. | Our results show that the composite RCNA+YFP generates fluorescence in the presence of cobalt. Furthermore, it can be used to distinguish between ischemic and non ischemic individuals. Further characterization, including usage of samples containing human IMA (ischemia modified albumin) and normal albumin, is needed, in order to improve our composite’s documentation. | ||
- | Regarding TMAO, we found a 3 hour-peak of fluorescence on the bacterial growth exponential phase once more. Higher concentration stimuli presented best results, although concentrations over 10mM seem to saturate PSB1C3_TorCAD promoter at this phase (figures 11 and 12). Further characterization using human sera is also needed for better composite’s documentation. | + | Regarding TMAO, we found a 3 hour-peak of fluorescence on the bacterial growth exponential phase once more. Higher concentration stimuli presented best results, although concentrations over 10mM seem to saturate PSB1C3_TorCAD promoter at this phase (figures 11 and 12). Further characterization using human sera is also needed for better composite’s documentation. --> |
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Revision as of 20:59, 27 October 2013