Team:TU-Munich/Results/KillSwitch

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[[File:TUM13_Foto_Labpics2.jpg|thumb|right|500px| '''Figure 3''': Working with the photosensitive protoplasts]]
[[File:TUM13_Foto_Labpics2.jpg|thumb|right|500px| '''Figure 3''': Working with the photosensitive protoplasts]]
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[http://www.plant-biotech.net/paper/CurrGenet_2003_hohe.pdf Hohe et al., 2003] "Targeted gene-knockout in Physco" paper

Revision as of 21:18, 28 October 2013


Kill Switch

The Kill Switch mechanism is the most complex and ambitious aspect of our project on the protein level. The DNA constructs are very large and even exceed the Part Registry´s frame (Fig. 1). For details on its design and function see here. We were very excited to start

Figure 1: [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159118 Registry entry] of the PhyB/PIF6 version of the Kill Switch, BBa_K1159118
File:PBI 376 f1.gif
Figure 2: Comparative expression performance of different constitutive mammalian and plant promoters in Physcomitrella patens, http://www.ncbi.nlm.nih.gov/pubmed/19021876 Gitzinger et al., 2009


Figure 3: Working with the photosensitive protoplasts

[http://www.plant-biotech.net/paper/CurrGenet_2003_hohe.pdf Hohe et al., 2003] "Targeted gene-knockout in Physco" paper


  • in bakkis nicht exprimiert [codon usage]
  • promotor zu stark [bild vgl prom stärken]
  • konstrukt zu groß für trafo [homologe regionen??]
  • filter nicht optimal [bild filter, ingmar @ blaulicht bench?]

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