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Team:TU-Eindhoven/LabJournal
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==Lab Journal== | ==Lab Journal== | ||
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{{:Team:TU-Eindhoven/Template:Timeline | title=Item Title | day=24 | month=July | year=2013 }} | {{:Team:TU-Eindhoven/Template:Timeline | title=Item Title | day=24 | month=July | year=2013 }} | ||
===Creating Agar Plates=== | ===Creating Agar Plates=== | ||
Revision as of 12:04, 25 July 2013
Lab Journal
Creating Agar Plates
Before any real lab work could begin some supplies had to be created, including a selection of agar plates. We needed two different types of agar plates, some with ampicillin antibiotics and others with kanamycin antibiotics. To manage this we created 2 separate agar solutions, a 200mL mixture for the ampicillin plates and a 400mL mixture for the kanamycin plates. The protocol we followed for the creation of the agar solutions was as follows:
- Mix together the following amounts to create 200mL agar solution:
- 2g Peptones
- 2g NaCl
- 1g Yeast extract
- 3g Agar
- Fill the container up to 200mL with demineralised water.
- For a 400mL solution mix together:
- 4g Peptones
- 4g NaCl
- 2g Yeast extract
- 6g Agar
- Fill the container up to 400mL with demineralised water.
- Next the containers were autoclaved and allowed to cool, but not harden.
- Before pouring the plates the correct antibiotics were added. The concentration of the ampicillin antibiotics was 100ηg/µL and that of the kanamycin antibiotics was 30ηg/µL.
