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Team:TU-Eindhoven/LabJournal
From 2013.igem.org
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==Lab Journal== | ==Lab Journal== | ||
| - | {{:Team:TU-Eindhoven/Template:Timeline | title= | + | {{:Team:TU-Eindhoven/Template:Timeline | title=Creating agar plates | day=24 | month=July | year=2013 }} |
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Before any real lab work could begin some supplies had to be created, including a selection of agar plates. We needed two different types of agar plates, some with ampicillin antibiotics and others with kanamycin antibiotics. To manage this we created 2 separate agar solutions, a 200mL mixture for the ampicillin plates and a 400mL mixture for the kanamycin plates. The protocol we followed for the creation of the agar solutions was as follows: | Before any real lab work could begin some supplies had to be created, including a selection of agar plates. We needed two different types of agar plates, some with ampicillin antibiotics and others with kanamycin antibiotics. To manage this we created 2 separate agar solutions, a 200mL mixture for the ampicillin plates and a 400mL mixture for the kanamycin plates. The protocol we followed for the creation of the agar solutions was as follows: | ||
*Mix together the following amounts to create 200mL agar solution: | *Mix together the following amounts to create 200mL agar solution: | ||
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**Fill the container up to 400mL with demineralised water. | **Fill the container up to 400mL with demineralised water. | ||
*Next the containers were autoclaved and allowed to cool, but not harden. | *Next the containers were autoclaved and allowed to cool, but not harden. | ||
| + | *The following steps were all performed in the vicinity of a blue flame to ensure a sterile working environment. | ||
*Before pouring the plates the correct antibiotics were added. The concentration of the ampicillin antibiotics was 100ηg/µL and that of the kanamycin antibiotics was 30ηg/µL. | *Before pouring the plates the correct antibiotics were added. The concentration of the ampicillin antibiotics was 100ηg/µL and that of the kanamycin antibiotics was 30ηg/µL. | ||
| + | *The solutions were now ready to be poured into agar plates. From the solutions we made we were able to pour 8 ampicillin plates and after receiving a little extra (about 350mL) kanamycin agar solution we poured a total of 22 kanamycin plates. | ||
| + | *The plates were cooled on the bench and allowed to harden before being stored in a 4° refrigerator. | ||
| + | |||
| + | {{:Team:TU-Eindhoven/Template:Timeline | title= Transforming the DNA| day=24 | month=July | year=2013 }} | ||
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{{:Team:TU-Eindhoven/Template:TimelineEnd}} | {{:Team:TU-Eindhoven/Template:TimelineEnd}} | ||
{{:Team:TU-Eindhoven/Template:Timeline | title=Item Title 2 | day=25 | month=July | year=2013 }} | {{:Team:TU-Eindhoven/Template:Timeline | title=Item Title 2 | day=25 | month=July | year=2013 }} | ||
Revision as of 12:11, 25 July 2013
Lab Journal
Before any real lab work could begin some supplies had to be created, including a selection of agar plates. We needed two different types of agar plates, some with ampicillin antibiotics and others with kanamycin antibiotics. To manage this we created 2 separate agar solutions, a 200mL mixture for the ampicillin plates and a 400mL mixture for the kanamycin plates. The protocol we followed for the creation of the agar solutions was as follows:
- Mix together the following amounts to create 200mL agar solution:
- 2g Peptones
- 2g NaCl
- 1g Yeast extract
- 3g Agar
- Fill the container up to 200mL with demineralised water.
- For a 400mL solution mix together:
- 4g Peptones
- 4g NaCl
- 2g Yeast extract
- 6g Agar
- Fill the container up to 400mL with demineralised water.
- Next the containers were autoclaved and allowed to cool, but not harden.
- The following steps were all performed in the vicinity of a blue flame to ensure a sterile working environment.
- Before pouring the plates the correct antibiotics were added. The concentration of the ampicillin antibiotics was 100ηg/µL and that of the kanamycin antibiotics was 30ηg/µL.
- The solutions were now ready to be poured into agar plates. From the solutions we made we were able to pour 8 ampicillin plates and after receiving a little extra (about 350mL) kanamycin agar solution we poured a total of 22 kanamycin plates.
- The plates were cooled on the bench and allowed to harden before being stored in a 4° refrigerator.
