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Team:Evry/Notebook/w1
From 2013.igem.org
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<h1>Week 1: 17th June - 23rd June</h1> | <h1>Week 1: 17th June - 23rd June</h1> | ||
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| + | <h2>Tuesday, 19th June</h2> | ||
| + | To make competent cells, we grew from a glycerol three E. coli strains in 2 mL of LB medium.<br/> | ||
| + | We chose for the following chemically competent strains:<br/> | ||
| + | BL21<br/> | ||
| + | DH5α<br/> | ||
| + | TOP10<br/> | ||
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<h2>Friday, 21st June</h2> | <h2>Friday, 21st June</h2> | ||
Revision as of 14:38, 28 July 2013
Week 1: 17th June - 23rd June
Tuesday, 19th June
To make competent cells, we grew from a glycerol three E. coli strains in 2 mL of LB medium.We chose for the following chemically competent strains:
BL21
DH5α
TOP10
Friday, 21st June
The tree following E. coli strains have been prepared to be chemically competent: BL21, DH5α and TOP10.
To check the quality of our work as well as their competence, each strain has been plated on LB medium with ampicillin, kanamycin or chloramphenicol. We also transformed our bacteria with a random plasmid and plated them on LB medium with ampicillin only. We incubated our petri dishes for the week end at 30°c
