Team:Leeds/Results

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Experimental Results

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Here are Our Results from the lab so far

Bacterial growth curves

We are monitoring the growth of our bacterial devices to see if any of the genes we have inserted have an effect on bacterial growth.

Control Bacterial Growth Curve

This growth curve shows the lag phase and log phase of untransformed bacteria. The graph also shows that the bacteria do not grow in the presence of chloramphenecol. Our bacterial device will grow in the presence of chloramphenecol. We will use this graph as a comparison with our bacterial device.

Agarose Gels For Digestions

For every Digestion we do we will run a gel to ensure the digestion was successful, and use gel extraction kits to obtain purified DNA.

Digestion of plasmid containing Green Flourescent Protein

The part [http://parts.igem.org/Part:BBa_K081012:Design K081012] was digested with Ecor1 and a gel run to make sure the fragment(s)are of the correct length.

Agarose gel of GFP Digestion with Ecor1

This gel shows the bands obtained by Ecor1 digestion of BBa-K081012. There are 100bp ladders and 1kbp ladders. This gel was used to work out the size of the fragments obtained.

DNA Ladder Calibration Graph

From this graph we can work out that one fragment is around 3000bp long the other appears to be longer than the plasmid so we can assume this is undigested plasmid. The plasmid is 2858bp long. Through digestion it has been linearised by a single Ecor1 digest. The fragment corresponds to what we expect so our digest was succesful. Although not all of the plasmid was digested.

Geneious, our fine sponsors and suppliers of software

Bioline, our fine sponsors and suppliers of equipment

Qiagen, our fine sponsors and suppliers of PCR kits

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 ===DNA Ladder Calibration Graph===
-[[File:Leeds_DNAladdergraph.png|right|300px|DNA Ladder Calibration Graph for GFP Digestion|frameless]]From this graph we can work out that one fragment is around 3000bp long the other appears to be longer than the plasmid so we can assume this is undigested plasmid. The plasmid is 2858bp long. Through digestion it has been linearised by a single Ecor1 digest. The fragment corresponds to what we expect so our digest was succesful. Although not all of the plasmid was digested.
+[[File:Leeds_DNAladdergraph.png|right|300px|DNA Ladder Calibration Graph for GFP Digestion|frameless]]From this graph we can work out that one fragment is around 3000bp long the other appears to be longer than the plasmid so we can assume this is undigested plasmid. The plasmid is 2858bp long. Through digestion it has been linearised by a single Ecor1 digest. The fragment corresponds to what we expect so our digest was succesful. Although not all of the plasmid was digested.}}