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Team:BYU Provo/Notebook/SmallPhage/Summerexp
From 2013.igem.org
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| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> During these couple weeks, we tried another 2 rounds of mutagenesis. The 5th round didn't work well so we made some major adjustments for the 6th round of mutagenesis. We believe that we were successful in finally mutating the phage and are giving them to the phage purification team to run a cesium chloride gradient on them. </font> |
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Revision as of 20:18, 5 August 2013
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July 1 - July 15
A new term and a fresh start! During these two weeks, we performed two more rounds of mutagenesis. With every experiment, we were able to perfect our protocol. Although our results are still not satisfactory, we are getting close! Eventually, we discovered that our phage stock decreased in titer during its preservation inside the fridge. We will propagate our phage and try again!
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| July 16 - July 31
During these couple weeks, we tried another 2 rounds of mutagenesis. The 5th round didn't work well so we made some major adjustments for the 6th round of mutagenesis. We believe that we were successful in finally mutating the phage and are giving them to the phage purification team to run a cesium chloride gradient on them.
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| August 1 - August 16
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