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Team:Paris Saclay/Notebook/August/8
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===='''Preparation of the PSB3K3 backbone plasmid'''==== | ===='''Preparation of the PSB3K3 backbone plasmid'''==== | ||
| - | =====Electrophoresis of BBa_J004450 digested by EcoRI/Pst1===== | + | =====1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right===== |
Damir, Nadia | Damir, Nadia | ||
| - | + | '''Experiment''' | |
| + | |||
| + | {| | ||
| + | | style="width:350px;border:1px solid black;" | IMAGE | ||
| + | | style="width:350px;border:1px solid black;" | | ||
| + | Well 1 : 6µL DNA Ladder | ||
| + | Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1 | ||
| + | Gel : 0.8% | ||
| + | |} | ||
| + | |||
| + | '''Analysis''' | ||
| + | |||
| + | Expected sizes : | ||
| + | |||
| + | PSB3K3 : 2750kb | ||
| + | GFP : 1069kb | ||
| + | |||
| + | We obtained fragments of the right size. | ||
| + | |||
| + | =====2 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to purify the pl===== | ||
| + | Damir, Nadia | ||
'''Experiment''' | '''Experiment''' | ||
Revision as of 14:26, 9 August 2013
Contents |
Notebook : August 8
Lab work
Aerobic/Anaerobic regulation system
Preparation of the PSB3K3 backbone plasmid
1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right
Damir, Nadia
Experiment
| IMAGE |
Well 1 : 6µL DNA Ladder Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1 Gel : 0.8% |
Analysis
Expected sizes :
PSB3K3 : 2750kb GFP : 1069kb
We obtained fragments of the right size.
2 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to purify the pl
Damir, Nadia
Experiment
| IMAGE |
Well 1 : 6µL DNA Ladder Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1 Gel : 0.8% |
Analysis
Expected sizes :
PSB3K3 : 2750kb GFP : 1069kb
We obtained fragments of the right size.
