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Team:Paris Saclay/Notebook/August/6
From 2013.igem.org
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Expected size : 500 bp | Expected size : 500 bp | ||
| - | We obtained fragments of the right size. Our backbone contains the Biobrick. | + | We obtained fragments of the right size. Our backbone contains the Biobrick. |
*NirB promoter in psB1C3 | *NirB promoter in psB1C3 | ||
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Expected size : 500 bp | Expected size : 500 bp | ||
| - | We obtained fragments of the right size. Our backbone contains the Biobrick. | + | We obtained fragments of the right size. Our backbone contains the Biobrick. |
*NarG promoter in psB1C3 | *NarG promoter in psB1C3 | ||
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Expected size : 500 bp | Expected size : 500 bp | ||
| - | We obtained fragments of the right size. Our backbone contains the Biobrick. | + | We obtained fragments of the right size. Our backbone contains the Biobrick. |
====2 - Samples culture==== | ====2 - Samples culture==== | ||
Revision as of 12:22, 11 August 2013
Contents |
Notebook : August 6
Lab work
A - Aerobic/Anaerobic regulation system
Obtaining activator FNR promoter (narK, nirB, narG)
1 - Gel electrophoresis of the colony PCR products ( made the 08/05/2013)on 25 colonies
XiaoJing, Damir, Anaïs
- NarK promoter in psB1C3
| IMAGE |
|
Expected size : 500 bp
We obtained fragments of the right size. Our backbone contains the Biobrick.
- NirB promoter in psB1C3
| IMAGE |
|
Expected size : 500 bp
We obtained fragments of the right size. Our backbone contains the Biobrick.
- NarG promoter in psB1C3
| IMAGE |
|
Expected size : 500 bp
We obtained fragments of the right size. Our backbone contains the Biobrick.
2 - Samples culture
Xiaoing, Anaïs
We put in culture the 6, 7, 8 samples for every promoter in 5 ml LB + 5 µl Chlorenphenicol (1000x,20µg/ml) Incubator over night to 310,15K at 180 RPM
