KillSwitch

From 2013.igem.org

(Difference between revisions)

Revision as of 10:15, 16 August 2013

Kill Switch

The kill switch design is based on the expression of holin and antiholin. Holin is a protein that forms pores in cell membranes. Anti-holin binds to holin and inhibits it's action. Once pores are formed by holin, lysozyme can access the periplasmic space and degrade the cell wall, causing cell lysis.

@@ Line 6: / Line 6: @@
 <div style="margin-top:10px;margin-left:30px;margin-right:30px;float:left;display:inline-block;">
-<h2 align="center">Timer Plus Sumo</h2>
+<h2 align="center">Kill Switch</h2>
 <br>
-<p align="justify">In this section the system of Figure 1 is modeled. The structure of the timer is very similar version of the timer compared to the construct of iGEM TU Delft team 2009. Here the input is changed to a
+<p align="justify">
-T7 promoter and the output to Ulp-1. Furthermore, the Ulp-1 cleaves off the SUMO from the peptide
+The kill switch design is based on the expression of holin and antiholin. Holin is a protein that forms pores in cell membranes. Anti-holin binds to holin and inhibits it's action.  Once pores are formed by holin, lysozyme can access the periplasmic space and degrade the cell wall, causing cell lysis.
-combined with the SUMO.
 </p>
+</p>