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Team:Paris Saclay/Notebook/August/19
From 2013.igem.org
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*Well 1 : 6µL DNA Ladder | *Well 1 : 6µL DNA Ladder | ||
| - | *Well 2 : 5µL DNA + | + | *Well 2 : 5µL DNA + 1µl of 6X loading dye |
*Gel : 1% | *Gel : 1% | ||
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Revision as of 17:12, 20 August 2013
Contents |
Notebook : August 19
Lab work
A - Aerobic/Anaerobic regulation system
Objective : to obtain ...
1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI and PstI
Nadia
Protocol : Gel purification
Nanodrop :
- RBS-LacZ-Term : 59.6ng/µL
- RBS-AmilCP-Term : 21.3ng/µL
Concentrations are very low so we try to precipitate the DNA again.
2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term
Nadia, XiaoJing
Protocol : EtOH precipitation
We used 20µL of DNA.
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : to obtain ...
1 - Electrophoresis gel of BphR2 produce by PCR
Damir
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We obtain the BphR2 by PCR.
2 - Gel purification of BphR2
Damir
Protocol : Gel purification
Nanodrop :
- BphR2 : 57.2ng/µL
CONCLUSION
Meeting with Evry
