Team:Imperial College/Results

From 2013.igem.org

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| [[File:Very_stressed_ecoli.jpg|thumbnail|left|400px|Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated [https://2013.igem.org/Team:Imperial_College/Protocols WCM]]]
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| [[File:Very_stressed_ecoli.jpg|thumbnail|left|400px|Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated [https://2013.igem.org/Team:Imperial_College/Protocols WCM]]] for 48 hours.
| [[File:WCM_media.png|thumbnail|right|400px|Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC]]
| [[File:WCM_media.png|thumbnail|right|400px|Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC]]
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<b>Ethylene glycol growth assay</b>
<b>Ethylene glycol growth assay</b>
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[[File:EG_growth.png|thumbnail|center|600px|Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0mM, 100mM and 200mM Ethylene Glycol at 30ºC.]]
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[[File:EG_growth.png|thumbnail|center|600px|Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0mM, 100mM or 200mM Ethylene Glycol at 30ºC.]]
<b><span style="color:#e00">red</span>
<b><span style="color:#e00">red</span>

Revision as of 09:07, 23 August 2013

Results


Glucose growth assay

Cell growth of phaABC E. coli at 4 concentrations of glucose. Optimum growth is at 2-4% glucose at 37ºC.



WCM growth assay

Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated WCM
for 48 hours.
Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC



Ethylene glycol growth assay

Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0mM, 100mM or 200mM Ethylene Glycol at 30ºC.

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