Team:Imperial College/data
From 2013.igem.org
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| [[File:Very_stressed_ecoli.jpg|thumbnail|left|400px|Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated [https://2013.igem.org/Team:Imperial_College/Protocols WCM] for 48 hours.]] | | [[File:Very_stressed_ecoli.jpg|thumbnail|left|400px|Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated [https://2013.igem.org/Team:Imperial_College/Protocols WCM] for 48 hours.]] | ||
| [[File:WCM_media.png|thumbnail|right|400px|Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC. Error bars represents SE, n=4]] | | [[File:WCM_media.png|thumbnail|right|400px|Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC. Error bars represents SE, n=4]] | ||
- | |[[File:Stress_cells.jpg]] | + | |- |
+ | |[[File:Stress_cells.jpg|thumbnail|left|400px|Cells containing mCherry pigment grown in SRF over 3 days]] | ||
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Revision as of 09:40, 27 August 2013
Data
Glucose growth assay
WCM growth assay
Originally we intended on using [http://parts.igem.org/Part:BBa_K639003 BBa_K639003] to detect whether our cells were stressed when placed in various toxic byproducts. However, as the data below shows, this biobrick is very leaky. As such, we are using the stress sensor as a marker for cell growth and also to show that the cells had been successfully transformed with the correct chloramphenicol resistance.
Ethylene glycol growth assay