Team:TU-Munich/Results/Software/FeatureList

From 2013.igem.org

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Revision as of 20:09, 1 September 2013


Tag and binding sites

  • Arg5-tag: RRRRR
  • Strep-tag I: AWRHPQFGG
  • Strep-tag II: WSHPQFEK
  • Flag-tag: DYKDDDDK
  • 3xFlag-tag: DYKDHDGDYKDHDIDYKDDDDK
  • HA-tag: YPYDVPDYA
  • His5-tag: HHHHH
  • His6-tag: HHHHHH
  • c-Myc-tag: EQKLISEEDL
  • S-tag: KETAAAKFERQHMDS
  • HAT-tag: KDHLIHNVHKEFHAHAHNK
  • Calmodulin-binding-peptide: KRRWKKNFIAVSAANRFKKISSSGAL
  • Isopep-tag: TDKDMTITFTNKKDAE
  • Spy-tag: AHIVMVDAYKPTK
  • Chitin-binding domain: TNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ
  • SBP-tag: MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP
  • Avitag: GLNDIFEAQKIEWHE

Cleavage sites

  • Enterokinase cleavage site: DDDDK
  • Factor Xa cleavage site: ID/EGR
  • Thrombin cleavage site: LVPRGS
  • PreScission cleavage site: LEVLFQGP
  • TEV cleavage site: ENLYFQG
  • TVMV cleavage site: ETVRFQGS

Other features

  • RFC 25 scar: In-frame ACCGGC (in nucleotide sequence), translating to TG


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