Team:Paris Saclay/Notebook/August/19

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Contents

1 Notebook : August 19
- 1.1 Lab work
  - 1.1.1 A - Aerobic/Anaerobic regulation system
    - 1.1.1.1 Objective : to obtain ...
      - 1.1.1.1.1 1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI/PstI
      - 1.1.1.1.2 2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term
  - 1.1.2 A - Aerobic/Anaerobic regulation system / B - PCB sensor system
    - 1.1.2.1 Objective : to obtain ...
      - 1.1.2.1.1 1 - Electrophoresis gel of BphR2 produce by PCR
      - 1.1.2.1.2 2 - Gel purification of BphR2
- 1.2 Meeting with Evry

Notebook : August 19

Lab work

A - Aerobic/Anaerobic regulation system

Objective : to obtain ...

1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI/PstI

Nadia

Protocol : Gel purification

Nanodrop :

RBS-LacZ-Term : 59.6ng/µL
RBS-AmilCP-Term : 21.3ng/µL

Concentrations are very low so we try to precipitate the DNA again.

2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term

Nadia, XiaoJing

Protocol : EtOH precipitation Protocole ou à rédiger ici ?????????

We used 20µL of DNA.

Nanodrop :

RBS-LacZ-Term : 38.4ng/µL
RBS-AmilCP-Term : 28.7ng/µL

CONCLUSION

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : to obtain ...

1 - Electrophoresis gel of BphR2 produce by PCR

Damir

Well 1 : 6µL DNA Ladder
Well 2 : 5µL DNA + 1µl of 6X loading dye
Gel : 1%

Expected size :

BphR2 Part I : 178 kb

We obtain a frangment at the roght size. We can purify it.

2 - Gel purification of BphR2

Damir

Protocol : Gel purification

Nanodrop :

BphR2 : 57.2ng/µL

CONCLUSION

Meeting with Evry

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