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Team:Paris Saclay/Notebook/August/19
From 2013.igem.org
Contents |
Notebook : August 19
Lab work
A - Aerobic/Anaerobic regulation system
Objective : to obtain ...
1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI/PstI
Nadia
Protocol : Gel purification
Nanodrop :
- RBS-LacZ-Term : 59.6ng/µL
- RBS-AmilCP-Term : 21.3ng/µL
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Concentrations are very low so we try to precipitate the DNA again. |
2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term
Nadia, XiaoJing
Protocol : EtOH precipitation Protocole ou à rédiger ici ?????????
We used 20µL of DNA.
Nanodrop :
- RBS-LacZ-Term : 38.4ng/µL
- RBS-AmilCP-Term : 28.7ng/µL
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CONCLUSION |
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins (Gibson assembly)
1 - Electrophoresis gel of PCR products : BphR2 Part I
Damir
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Expected size :
- BphR2 Part I : 178 kb
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We obtain a frangment at the right size. We can purify it. |
2 - Gel purification of PCR products : BphR2 Part I
Damir
Protocol : Gel purification
Nanodrop :
- BphR2 : 57.2ng/µL
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CONCLUSION |
Meeting with Evry
