Team:Paris Saclay/gel

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Electrophoresis on gel

Gels are made with agarose in pounder with TAE 0.5X

To make 1% gel:

1. Put 1g of agarose in pounder

2. Add TAE until 100mL

3. Boil until the solution become uniform

4. Add BET with a final concentration of 200ng/µL

5. Pour gels in molds

6. Let agarose in suffusion at 60°C

Put the misted DNA with charged blue diluted 6X