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Team:INSA Toulouse/contenu/lab practice/notebook/calendar/recomb
From 2013.igem.org
Calendar
Recombinases
July 2013
- Week 6 (15-21 July)
15/07
Assembling Bxb1 with terminator by 3A assembling into pSB1A3
Assembling FimE recombinase with rbs by 3A assembling into pSB1C3
Mutagenese of PhiC31 from the Suiti Parts (A24) to delete the EcoRI restriction site
PCR to extract Tp901.1 from the Bonnet parts
PCR to invert RFP and rbs.LuxI with the restriction site ClaI BamHI
16/07
Purification of the PCR and verification by gel.
Miniprep for the verification of the clonage, Bxb1.term and rbs.FimE.
For the mutagenese of PhiC31, restriction by DpnI and transformation.
17/07
Assembling Tp901.1 with terminator by 3A assembling.
PCR of extraction of the vector A24 with Phic31 mutated.
18/07
Assembling PhiC31 mutated with the terminator into pSB1C3.
19/07
Assembling of rbs.LuxI and cI for the final system with the AND1 gate.
Assembling of rbs.FimE with terminator into pSB1C3.
Arrival of the synthesis.
20/07
Verification of the clonage.
Assembling the synthesis parts (the 4 gates and the 6 riboregulators) into pSB1C3.
- Week 7 (22-28 July)
22/07
Assembling XOR1 with PhiC31*, LuxI with cI and the riboregulator 1, 4, 5...
August 2013
- Week ...
- Week ...
