Team:Paris Saclay/Notebook/July/12

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Notebook : July 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155003, Bba_K1155007

1 -Transformation of Bba_I732019, Bba_B0015, Bba_B0017, Bba_B0010

Zhou

Transformation of 07/10/13 of Bba_I732019 didn't works. We will do it again. We obtain two colonies of 07/10/13 transformation. We will extract Bba_B0010 and do the transformation again.

Protocol : Bacterial transformation

2 -Design of RBS-AmilCP oligos

Abdou, Anaïs

We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.

3 -Extraction of Bba_B0010 from DH5α

Sheng

Protocol : High copy plamid extraction


B - PCB sensor system

Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2

1 - Stock of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2

Zhou

Used quantities :

  • Glycerol 85% : 425
  • Culture : 1µL

2 - Extraction of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2 from DH5α

Abdou, Sheng

Protocol : High copy plamid extraction

3 - Streak of BphA1 and BphR2

Anaïs

We strek 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.

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