"
Team:UC Davis/Parts
From 2013.igem.org
Parts
The following parts were assembled throughout the development of this project:| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1212002 | Plasmid_Backbone | Golden Gate Compatible pSB3K3 | Alex Neckelmann | 2750 |
| BBa_K1212006 | Composite | pBAD+Golden Gate Compatible GFP | Aura Ferreiro | 1052 |
| BBa_K1212009 | Composite | pBAD+Riboswitch 2+GFP+T | Amy Soon | 1086 |
| BBa_K1212010 | Composite | pBAD+Riboswitch 1+GFP+T | Amy Soon | 1090 |
| BBa_K1212011 | Composite | pBAD+Riboswitch 2+TAL1 | Amy Soon | 2612 |
| BBa_K1212012 | Composite | pBAD+Riboswitch 2+TAL8 | Amy Soon | 2616 |
| BBa_K1212013 | Composite | pTet+TBS 1+RBS+GFP+T | Aura Ferreiro | 1003 |
| BBa_K1212014 | Composite | pBAD+Riboswitch 1+TAL 1 | Amy Soon | 2616 |
| BBa_K1212015 | Composite | pBAD+Riboswitch 1+TAL 8 | Amy Soon | 2620 |
| BBa_K1212016 | Composite | pTet+TBS 2+RBS+GFP | Amy Soon | 1003 |
Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (BBa_K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization.
Project BackgroundLearn about how we combine riboswitches and TALs into robust orthogonal mechanisms for inducible repression. |
ResultsCheck out the results of our experiments. |
Human PracticesTake a look at how we designed a new database for better raw data characterization of Biobricks. |
Judging CriteriaHere's the criteria that we met for this year's team. |
