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Team:Evry/ChemicalTools
From 2013.igem.org
Chemical tools
There are 6 enzyms involved in the natural process of the enterobactin production:
- EntA :
- EntB :
- EntC :
- EntD :
- EntE :
- EntF :
For now, we consider each one of these steps as a simple chemical reaction:
We are using the enzymatic kinetic model of Michaelis-Menten, which divides each reaction into two consecutives steps:
The speed of the reaction is calculated as below:
The steady state approximation gives us:
And thus,
where 
and 
are classic kinetic parameters.
Conclusion:
For simple enzymatic reactions (one reagent, one product and one enzyme) with the steady state approximation, we can directly apply the formula above-written.
Site developped by Gabriel Guillocheau
