Team:TU-Eindhoven/Results

Aerobic Expression

In the experiments for the aerobic expression of the proteins, we have seen that we have produced functional Biobricks. The proteins 1ETF, 1PJN, 1G70 and EGFP were produced aerobically so that we can say that the following Biobricks were succesfull: [http://parts.igem.org/Part:BBa_K1123015 BBa_K11230014], [http://parts.igem.org/Part:BBa_K1123015 BBa_K1123015], [http://parts.igem.org/Part:BBa_K1123016 BBa_K1123016] and [http://parts.igem.org/Part:BBa_K1123015 BBa_K1123017]. For more detailed information and further results obtained by these experiments, see CEST Agent Testing.

CEST Agent Testing

Anaerobic FNR Testing

After analyzing the samples taken during anaerobically induced expression of EGFP, we can conclude that the FNR promoter is a functional Biobrick. We can conclude that the EGFP Protein Production Construct is functional too. WE performed Fluorescence measurments of the samples taken at different time points during the anaerobically induced expression of EGFP.

These experiments resulted in the observation that we have two more functional Biobricks: [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1123000 BBa_K1123001] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1123005 BBa_K1123005]. To see detailed information and results, see Anaerobic FNR Testing.