Team:TU-Munich/Results/Overview

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Results Overview

Having spent our summer in the lab, we are proud to present our accomplishments. Previous to the Euopean regional jamboree in Lyon, we have created 72 BioBricks and devices, transformed and selected 20 different transgenic GM-mosses and characterized our effectors as recombinant proteins produced in E. coli and in our moss Physcomitrella patens, a chassis newly introduced into iGEM. We took further steps to put our phytoremediation project into practise by developing concepts for the implementation of our PhyscoFilter in the environment and by analyzing the economic potential of this innovative technology in our Entrepreneurship section. Additional to our wetlab work, we contributed a very powerful software tool for the annotation of BioBricks from the parts registry and contributed tutorials to pass this summer´s experiences and skills on to the subsequent iGEM generations.

BioBricks

We created 72 BioBricks, including Bricks advancing the use of Physcomitrella patens as a chassis, Bricks supplying effectors for phytoremediation applications and BioBricks for the light triggered kill-switch mechanism. (Read more)

Effectors

We chose six different effector proteins which we have produced recombinantly in Escherichia coli to characterize them on a molecular level concerning stability and activity.
Read more

WT-Moss

Due to no research on Physcomitrella patens at our university, we had to make some general experiments with this moss concerning tolerance of toxin, growing properties on different surfaces and the determination of growth rates.
Read more

GM-Moss

In our project we created 25 different strains of stabily transformated moss, which was done 350 km away in Freiburg. We then selected and regenerated transgenic moss over 4 weeks and tested several of them in 96-well plates.
Read more

Software

Protein coding BioBricks are an important point in the Parts Registry. As they are standardized by definition, our AutoAnnotator translates the sequence, computes different valuable parameters, does alignments and presents all these information as a formatted table. Read more

RFC 96

To improve the standardization of protein-encoding Biobricks, we introduce the AutoAnnotator, a software tool that calculates several information by using the DNA sequence of the Biobricks as a blueprint to display them in a standard table.
Read more

Tutorials

During the summer we learned a lot about iGEM and found solutions for problems which appear in many iGEM projects. So we want to share this knowledge for the benefit of the community. Therefore we created our tutorial section.
Read more

Entrepreneurship

Science is an extremely important point for progress, but in the end it is essential to develop a business model oppon which the technology can be brought to market.
Read more

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