Team:TU-Munich/Results/Overview

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Results Overview

Having spent our summer in the lab, we are proud to present our accomplishments. Upto the European regional jamboree in Lyon, we have created 72 BioBricks and devices, transformed and selected 20 different transgenic GM-mosses and characterized our effectors as recombinant proteins produced in E. coli and in our moss Physcomitrella patens, a chassis newly introduced into iGEM. We took further steps to put our phytoremediation project into practice by developing concepts for the implementation of our PhyscoFilter in the environment and by analyzing the economic potential of this innovative technology in our Entrepreneurship section. Additional to our wetlab work, we contributed a very powerful software tool for the annotation of BioBricks from the parts registry and contributed tutorials to pass this summer´s experiences and skills on to the subsequent iGEM generations.

BioBricks

We created 72 BioBricks, including BioBricks advancing the use of Physcomitrella patens as a chassis, BioBricks enhancing phytoremediation applications and BioBricks for the light triggered kill-switch mechanism. (Read more)

Effectors

We selected six different effector proteins and produced them in Escherichia coli and further characterized them concerning stability and activity. (Read more)

WT-Moss

We performed some general experiments to optimize Physcomitrella patens concerning tolerance of toxins, growth optimization and the use of different cultivation surfaces. (Read more)

GM-Moss

We created 20 different strains of transformated moss during our visit to Prof. Reski´s lab in Freiburg. After selection and regeneration transgenic, we started the experiments. (Read more)

Software

Protein coding BioBricks constitute large parts of the Parts Registry. We created the AutoAnnotator for convenient in-silico translation of their sequences and provision of various valuable parameters summed up in a formatted table. (Read more)

RFC 96

For the improvement of the parts registry, the RFC 96 standard proposes a range of characteristics convenietly determined by the AutoAnnotator, to describe the coding BioBricks details. (Read more)

Tutorials

We would like to share the experiences we made and pass on the solutions and skills we generated this summer with the iGEM community, so we created a couple of useful tutorials for the following iGEM generations. (Read more)

Entrepreneurship

To translate science into applied technology available to the public, economic and business factors play increasing roles. We took the first steps into this direction by examining criteria for implementation and possibilities for business models in biotechnology. (Read more)

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