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Team:Evry/pop scale
From 2013.igem.org
Introduction
The Enterobactin production model showed us that our bacteria take too much time to produce enterobactins, which disables the flush strategy.
In response, we changed the strategy, by delivering a gel that would block the bacteria in the jejunum.
This model is thus very similar to the flush treatment model, except for the longer time scale, and the computational method.
Goals
Assumptions
- No regulation of the patient's iron absorption
- Constant iron flow in the intestine
- Homogeneous fluid
- The bacterial natural absorption is insignificant compared to the chelation
- The patient ingests 20mg of iron per day (Guideline Daily Amounts)
Materials and methods
This model is based on a cellular automaton algorithm : both the bacteria and the enterobactins are cellular automaton.
Figure 1 : Influence graph.
The Figure 1 represents the influence between the system's variables.
Figure 2 : Graph of bacteria automaton.
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La Figure 2 représente l'automate qui modélise la bactérie. Cet automate à deux parties : la production d'enterobactines et la croissance.
The bacteria produces enterobactins. This production is ruled by a activator. Cette activateur est une fonction palier de 0 à 1 avec un seuil Ka fixer grace au modèle du senseur, dont voici l'expression :
Enterobactin chelate iron. For this automoton, we have one rules : One Enterobactin atom can chelate one Iron Ion
L'automate est tout de même conditionné par un une probabilité de rencontre :
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Parameters tuning :
Results
Parameter values
| Name | Value | Unit | Description | Reference |
| mu | 0.2 | s-1 | Iron absorption rate by the body | |
| IronQuantity0 | 4.5.10-9 | mol-1 | Iron pulse value | |
| Sact | 10**-9 | m | Duodenum length | [3] |
| α | 0.3 | s-1 | Duodenum absorption rate | tuned |
| σ | 0.72 | s-1 | Regulation coefficient | tuned |
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