Team:Paris Bettencourt/Notebook

Team:Paris Bettencourt/Notebook

Week 01: 3^th - 9^th June

Week 02: 10^th - 16^th June

Week 03: 17^th - 23^th June

Week 04: 24^th - 30^th June

Week 05: 1^st - 7^th July

Week 06: 8^th - 14^th July

Week 07: 15^th - 21^th July

Week 08: 22^th - 28^th July

Week 09: 29^th July - 4^th August

Week 10: 5^th - 11^th August

Week 11: 12^th - 18^th August

Week 12: 19^th - 25^th August

Week 13: 26^th August - 1^st September

Week 14: 2^nd - 8^th September

Week 15: 9^th - 15^th September

Week 16: 16^th - 22^th September

Week 17: 23^th - 29^th September

Week 18: 30^th September - 6^th October

Week 19: 7^th - 13^th October

Week 20: 14^th - 20^th October

Week 21: 21^th - 27^th October

Week 22: 28^th October - 3^th November

Contents

Week 01: 3th - 9th June

Week 02: 10th - 16th June

Week 03: 17th - 23th June

Week 04: 24th - 30th June

Week 05: 1st - 7th July

Week 06: 8th - 14th July

Week 01: 3^th - 9^th June

Week 02: 10^th - 16^th June

Week 03: 17^th - 23^th June

Week 04: 24^th - 30^th June

Week 05: 1^st - 7^th July

Week 06: 8^th - 14^th July

Week 07: 15^th - 21^th July

Week 08: 22^th - 28^th July

From 2013.igem.org

Monday 3^th June

Tuesday 4^th June

Wednesday 5^th June

Thursday 6^th June

Friday 7^th June

Monday 10^th June

Tuesday 11^th June

Wednesday 12^th June

Thursday 13^th June

Friday 14^th June

Monday 17^th June

Tuesday 18^th June

Wednesday 19^th June

Thursday 20^th June

Friday 21^th June

Monday 24^th June

Tuesday 25^th June

Wednesday 26^th June

Thursday 27^th June

Friday 28^th June

Monday 01^st July

Tuesday 02^st July

Wednesday 03^st July

Thursday 04^st July

Friday 04^st July

Monday 8^th July

Tuesday 9^th July

Wednesday 10^th July

Thursday 11^th July

Friday 12^th July

Monday 15^th July

Tuesday 16^th July

Wednesday 17^th July

Thursday 18^th July

Friday 19^th July

Monday 22^th July

Tuesday 23^th July

Wednesday 24^th July

Thursday 25^th July

Friday 26^th July

From 2013.igem.org

Monday 3th June

Tuesday 4th June

Wednesday 5th June

Thursday 6th June

Friday 7th June

Monday 10th June

Tuesday 11th June

Wednesday 12th June

Thursday 13th June

Friday 14th June

Monday 17th June

Tuesday 18th June

Wednesday 19th June

Thursday 20th June

Friday 21th June

Monday 24th June

Tuesday 25th June

Wednesday 26th June

Thursday 27th June

Friday 28th June

Monday 01st July

Tuesday 02st July

Wednesday 03st July

Thursday 04st July

Friday 04st July

Monday 8th July

Tuesday 9th July

Monday 3^th June

Tuesday 4^th June

Wednesday 5^th June

Thursday 6^th June

Friday 7^th June

Monday 10^th June

Tuesday 11^th June

Wednesday 12^th June

Thursday 13^th June

Friday 14^th June

Monday 17^th June

Tuesday 18^th June

Wednesday 19^th June

Thursday 20^th June

Friday 21^th June

Monday 24^th June

Tuesday 25^th June

Wednesday 26^th June

Thursday 27^th June

Friday 28^th June

Monday 01^st July

Tuesday 02^st July

Wednesday 03^st July

Thursday 04^st July

Friday 04^st July

Monday 8^th July

Tuesday 9^th July

Wednesday 10^th July

Thursday 11^th July

Friday 12^th July

Monday 15^th July

Tuesday 16^th July

Wednesday 17^th July

Thursday 18^th July

Friday 19^th July

Monday 22^th July

Tuesday 23^th July

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Modeling

Drug Screening

Phage Sensor

Trojan Horse

TB-ception

Human Practices

Media Preparation

Glycerol Stocks

Plasmid Extraction

Media Preparation

Glycerol Stocks

Plasmid Extraction

Revision as of 14:01, 23 July 2013 by ClovisB (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

PROJECT
- OVERVIEW
- DETECT
- TARGET
- INFILTRATE
- SABOTAGE
ACHIEVEMENTS
HUMAN PRACTICE
SAFETY
COLLABORATION
- COLLABORATION
- SENSIGEM
NOTEBOOK
TEAM

@iGEM_Paris

<body>

Notebook

1 Week 01: 3^th - 9^th June
- 1.1 Monday 3^th June
- 1.2 Tuesday 4^th June
- 1.3 Wednesday 5^th June
- 1.4 Thursday 6^th June
- 1.5 Friday 7^th June
2 Week 02: 10^th - 16^th June
- 2.1 Monday 10^th June
- 2.2 Tuesday 11^th June
- 2.3 Wednesday 12^th June
- 2.4 Thursday 13^th June
- 2.5 Friday 14^th June
3 Week 03: 17^th - 23^th June
- 3.1 Monday 17^th June
- 3.2 Tuesday 18^th June
- 3.3 Wednesday 19^th June
- 3.4 Thursday 20^th June
- 3.5 Friday 21^th June
4 Week 04: 24^th - 30^th June
- 4.1 Monday 24^th June
- 4.2 Tuesday 25^th June
- 4.3 Wednesday 26^th June
- 4.4 Thursday 27^th June
- 4.5 Friday 28^th June
5 Week 05: 1^st - 7^th July
- 5.1 Monday 01^st July
- 5.2 Tuesday 02^st July
- 5.3 Wednesday 03^st July
- 5.4 Thursday 04^st July
- 5.5 Friday 04^st July
6 Week 06: 8^th - 14^th July
- 6.1 Monday 8^th July
- 6.2 Tuesday 9^th July
- 6.3 Wednesday 10^th July
- 6.4 Thursday 11^th July
- 6.5 Friday 12^th July
7 Week 07: 15^th - 21^th July
- 7.1 Monday 15^th July
- 7.2 Tuesday 16^th July
- 7.3 Wednesday 17^th July
- 7.4 Thursday 18^th July
- 7.5 Friday 19^th July
8 Week 08: 22^th - 28^th July
- 8.1 Monday 22^th July
- 8.2 Tuesday 23^th July
- 8.3 Wednesday 24^th July
- 8.4 Thursday 25^th July
- 8.5 Friday 26^th July
9 Week 09: 29^th July - 4^th August
10 Week 10: 5^th - 11^th August
11 Week 11: 12^th - 18^th August
12 Week 12: 19^th - 25^th August
13 Week 13: 26^th August - 1^st September
14 Week 14: 2^nd - 8^th September
15 Week 15: 9^th - 15^th September
16 Week 16: 16^th - 22^th September
17 Week 17: 23^th - 29^th September
18 Week 18: 30^th September - 6^th October
19 Week 19: 7^th - 13^th October
20 Week 20: 14^th - 20^th October
21 Week 21: 21^th - 27^th October
22 Week 22: 28^th October - 3^th November

Media was made, Strains sD001-sD004 were received, inoculated, and put into stock and catalog.

4 strains were received from Jake:

E. coli: BL21 (DE3) ko20 ΔcysI, Δfpr, ΔydbK
E. coli: NEBTurbo zmSIR Chloramphenicol
E. coli: NEBTurbo zmFNR Spectinomycin
E. coli: NEBTurbo soFD, zmSIR Chloramphenicol

Media and Glycerol stock were prepared:

3 500ml bottles of LB broth and LB agar were prepared by standard methods.
12.5g/500ml powder/water for broth and 20g/500ml powder/water for agar.
Bottles were autoclaved. Additionally 1 flask of 500ml of LB broth was made in the same fashion.

Single colonies from agar plates were picked and used to innoculate 5ml LB broth overnight.
750ml of overnight culture was added to 250ml of 60% glycerol in a cryotube.
Two sets of Glycerol stocks were used for each of sD001, sD002, sD003, sD004; one set was frozen at -20ºC and the other set was frozen at -80ºC.

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Plasmids were extracted from strains sD002, sD003, sD004

Plasmids pD004 (pCDF.ew12 zmFNR Spectinomycin), pD005 (pACYC.ew13 soFD, zmSIR Chloramphenicol), pD006 (pACYC.ew17 zmSIR Chloramphenicol) were extracted from NEBTurbo cells using a Thermo Scientific GeneJet Plasmid mini prep kit as described in the protocol (available on google drive).
Lacking Resuspention solution we used some from a different mini prep upstairs.
Plasmids were eluted in 100ul of nanopure water and frozen at -20ºC. Plasmids were included in catalog.

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Competent Cells : BL21 (DE3) dCysI dFpr dydbk

We grew 5ml of sD001 cells overnight from a single colony.
This 5ml was used to innoculate 500ml of LB broth.
Broth was incubated for 1.5h and optical density was read at 0.62 OD600.
Cells were alloquoted into 10 falcon tubes (50ml each) and centrifuged at 4000xg for 20 minutes at 4C.
Supernatant was removed and cells were resuspended in 200ml of Buffer 1 (4x 50ml) and left on ice for 20 minutes.
Cells were centrifuged again for 20 minutes at 4000xg.
Supernatant was removed and cells were resuspended in 12ml Buffer 2.
Cells were alloquoted at 500ul into microcentifuge tubes and frozen at -80C.

Buffer 1: 50mM CaCl2
Buffer 2: 0.53ml 2M CaCl2 2.8ml 60% Glycerol 8.67ml sterile H2O

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Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France

+33 1 44 41 25 22/25

team2013@igem-paris.org

July 2013
Mo	Tu	We	Th	Fr	Sa	Su
	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30	31

August 2013
Mo	Tu	We	Th	Fr	Sa	Su
			1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31

October 2013
Mo	Tu	We	Th	Fr	Sa	Su
	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30	31	1	2	3

June 2013