Team:TU-Munich/Notebook/Materials

From 2013.igem.org

Revision as of 13:26, 12 August 2013 by RCiccone (Talk | contribs)



Reagents

Chemicals

Reagent Supplier

Kits and Standards

Plastic Disposables

Oligonucleotides

Name Sequence
EreA_for 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘
EreA-rev 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘
EreA_SDMI_for 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘
EreA_SDMI_rev 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'
EreA_SDMII_for 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'
EreA_SDMII_rev 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'
EreA_SDMIII_for 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'
EreA_SDMIII_rev 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'
EreB_for 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'
EreB-rev 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'
EreB_SDMI_for 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'
EreB_SDMI_rev 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'
EreB_SDMII_for 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'
EreB_SDMII_rev 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'
EreB_SDMIII_for 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'
EreB_SDMIII_rev 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'
TEV_fw 5'- GCGCGTCTAGATGGCCGGCGGAGAAAGCTTGTTTAAGGGGC - 3'
TEV_rv 5'- ATATAACCGGTATTCATGAGTTGAGTCGCTTCC - 3'
TEV_t387c_fw 5'- ATGGTGTCAGACACTAGCTGCACATTCCCTTCATC - 3'
TEV_t387c_rv 5'- GATGAAGGGAATGTGCAGCTAGTGTCTGACACCAT - 3'
N_split_TEV_fw 5'- ATTCGCGGCCGCTTCTAG - 3'
N_split_TEV_rv 5'- TTCGAAACCGGTAGTTTGGAAGTTGGTTGTCAC - 3'
C_split_TEV_fw 5'- AAGCTTTCTAGATGGCCGGCAAGAGCATGTCTAGCATGGTG - 3'
C_split_TEV_rv 5'- CCGCTACTAGTATTAACCGG - 3'
PIF6_2-100_fw 5'- AAGCTTTCTAGATGGCCGGCATGTTCTTACCAACCGATTATTGTTGC - 3'
PIF6_2-100_rv 5'- TTCGAAACCGGTGTCAACATGTTTATTGCTTTC - 3'
MinMCS_fo 5'- CTAGACCTAGGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'
MinMCS_rev 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCCTAGGT - 3'
Bpu_for 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'
Bpu_rev 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'
Bpu_SDMI_for 5'- CACTTAAGCATTTTCTACGGGTCGATCACACCATTCA - 3'
Bpu_SDMI_rev 5'- TGAATGGTGTGATCGACCCGTAGAAAATGCTTAAGTG - 3'
Bpu_SDMII_fo 5'- GACACGATTAAATGTGTATGCGGGCTTAGCTGGATTTTATTTG - 3'
Bpu_SDMII_re 5'- CAAATAAAATCCAGCTAAGCCCGCATACACATTTAATCGTGTC - 3'
Bpu_SDMIII_fo 5'- CACCCTTAAAAATAAAGCTGGCTGCGGACAGGAAG - 3'
Bpu_SDMIII_re 5'- TTCCTGTCCGCAGCCAGCTTTATTTTTAAGGGTG - 3'
Bpu_SDMIV_fo 5'- CGTACGCTCACTCTTACGGGTACACAGGATAAATA - 3'
Bpu_SDMIV_re 5'- TATTTATCCTGTGTACCCGTAAGAGTGAGCGTACG - 3'
xylE_for 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3'
xylE_rev 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3'
xylE_c312a_for 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3'
xylE_c312a_rev 5'- GCGCACGCGCCGTCCACAACTGTTC - 3'
xylE_g483a_for 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'
xylE_g483a_rev 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'
xylE_a834g_for 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'
xylE_a834_rev 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'
p Act_for 5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'
pAct_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'
t35S_for 5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'
t35S_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'
Npt_for 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'
Npt_rev 5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'
miniMCSII_fw 5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'
miniMCSII_rv 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'
SpyTag_fw 5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3'
SpyTag_rv 5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3'
PIF3(2-100)_fw 5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3'
PIF3(2-100)_rv 5'- CCGCTACTAGTATTAACCGG - 3'
NucA_fw 5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3'
NucA_rv 5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3'
AlcR_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3'
AlcR_rv 5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3'
SQ_pAutoRex8_1 5'- CGGGGCATCACAGCGTCG - 3'
SQ_pAutoRex8_2 5'- TATCGGTAACCGAGTCCG - 3'
AlcR_QCI_fw 5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3'
AlcR_QCI_rv 5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3'
AlcR_QCII_fw 5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3'
AlcR_QCII_rv 5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3'
AlcR_QCIII_fw 5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3'
AlcR_QCIII_rv 5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3'
AlcR_QCIV_fw 5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3'
AlcR_QCIV_rv 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'
pActin_QC_fw 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'
pActin_QC_rv 5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3'
NPT_QCI_fw 5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3'
NPT_QCI_rv 5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3'
NPT_QCII_fw 5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3'
NPT_QCII_rv 5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3'
IRES_polio_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3'
IRES_polio_rv 5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3'
BPU_SDMI_r_for 5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3'
BPU_SDMI_r_rev 5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3'
Strep-TEV_for 5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3'
Strep-TEV_rev 5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3'
SQ_pluc-Actin_1 5'- CCGACTCAAATACAGATATGC - 3'
SQ_pluc-Actin_2 5'- CTCGGATGTAGATCTGCG - 3'
pActin_QC_ins_fw 5'- ACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACC
ACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACG - 3'
pActin_QC_ins_rv 5'- CGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGT
GGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGT - 3'
pActin_Oryza_fw 5'- AAGCTTTCTAGAGTAGCTAGCATACTCGAGGTCATTCATATGCTTG - 3'
pActin_Oryza_rv 5'- TTCGAACTGCAGCGGCCGCTACTAGTACTTCTACCTACAAAAAAGCTCCG - 3'
GGGGSx5_TEV_fw 5'- CTAGATGGCCGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGGAGG
CGGTGGCTCCGGAGGTGGAGGCTCAGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'
GGGGSx5_TEV_rv 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCTGAGCCTCCACCTCCGGAGCCACCGCCTCCC
GATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCGCCGGCCAT - 3'
mMCSII_fw 5'- AATTCGCGGCCGCTTCTAGAGCAATTGAAGCTTCCTGCAGGTA - 3'
mMCSII_rv 5'- CTAGTACCTGCAGGAAGCTTCAATTGCTCTAGAAGCGGCCGCG - 3'
FluA_tripmut_fw 5'- AAGCTTTCTAGATGGCCGGCGACGTGTACCACGACGGTG - 3'
FluA_tripmut_rv 5'- TTCGAAACCGGTATTGTTGACCTTGCAGGCGG - 3'
pActin_QCI_fw 5'- CATAATTTTTGAGAAAAATATATATTCAGGCGAATTGTCACAATGAACAATAATAAGATTAAAATA - 3'
pActin_QCI_rv 5'- TATTTTAATCTTATTATTGTTCATTGTGACAATTCGCCTGAATATATATTTTTCTCAAAAATTATG - 3'
Alc R fw Seq. 5'- CAGAGCACAATTGTCCATAC - 3'
Alc R rv Seq. 5'- TCGCGGTCGATGCTCTCC - 3'
pActin_QCins_fw 5'- CATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCAC
CACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTC - 3'
pActin_QCins_rv 5'- GAGGAGCTCGTCGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGG
TTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATG - 3'
PP1_for 5'- GCCGCTTCTAGATGGCCGGCGGATCCGCGGATTTAGATA - 3'
PP1_rev 5'- GTATTAACCGGTTTTCTTTGCTTGCTTTGTGATC - 3'
PhyB fw Seq 5'- GCGATTTCTCAGTTACAGGC - 3'
PhyB rv Seq. 5'- CTCAAAGCACGAGAAAGAAGC - 3'
pAct_ins_fw 5'- ACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTC - 3'
pAct_ins_rv 5'- GGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATGG - 3'
PppAct_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATCGAGTCCTTGTGACTTTTGTGCAT - 3'
PppAct_rv 5'- TTCGAAACTAGTATATTTAATACGGACCTGCACAACAACCACCAAT - 3'
PppActin_QC1_fw 5'- TTATTGAGGATATCCATCTATCTTGATTTTGGACAATGTTTTACTGC - 3'
PppActin_QC1_rv 5'- GCAGTAAAACATTGTCCAAAATCAAGATAGATGGATATCCTCAATAA - 3'
PppActin_QC2_fw 5'- ACACTCTACATTCATGTCAAGAGTATAGGGACTTGGG - 3'
PppActin_QC2_rv 5'- CCCAAGTCCCTATACTCTTGACATGAATGTAGAGTGT - 3'
PppActin_fw_corr 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGCGAGTCCTTGTGACTTTTGTGCAT - 3'
SYFP2_fw 5'- AAGCTTTCTAGATGGCCGGCGTTAGCAAGGGCGAAGAAC - 3'
SYFP2_rv 5'- TTCGAAACCGGTTTTATACAGCTCATCCATACCCAG - 3'
eCFP_fw 5'- AAGCTTTCTAGATGGCCGGCGTGAGCAAGGGCGAGGAG - 3'
eCFP_rv 5'- TTCGAAACCGGTCTTGTACAGCTCGTCCATGCC - 3'
G-TEV-site-G_fw 5'- CTAGATGGCCGGCGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'
G-TEV-site-G_fw 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCGCCGGCCAT - 3'
PppActin_seq_fw 5'- CATGTCCCAGGGCCCTAG - 3'

Instruments

Electrophoresis

Photometers

Nanodrop Spectrohpotometer

Moss-cultivation

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.


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        "TUM13_andreas-t.png",
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        "TUM13_polte-b-l.png",
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        "TUM13_polte-r.png",
        "TUM13_polte-t-r.png",
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       $members.eq(i).removeClass();
       $members.eq(i).addClass("top-right");

   } else if ( $members.eq(i).offset().top <= event.pageY && ( $members.eq(i).offset().top + $members.eq(i).height() ) >= event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("right");

   } else if ( ( $members.eq(i).offset().top + $members.eq(i).height() ) < event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("bottom-right");

   }

}

} });

}

});

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