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From 2013.igem.org

System Modeller light sensitivity model

This is the model that describes the detection of light output from our designed antibody/antigen complex. The model-program vill be incorporated into the physical biosensor to determine the amount of antigen in the sample.

This picture describes how the model will explain the antigen/antibody-complex binding and the light difference. As antibody is added to the sample (T=0 min) containing the unknown amount of HEWL-antigen green light is emitted from luciferase. When the RFP-antigen is added (T=10 min) the decrease in green fluorescence and the increase in red fluorescence will be detectable and the detector will thus give a signal saying if it is HEWL-antigen in the sample or not.