Notebook : July 3

Summary:

Observed the Petri dish, we decided to perform a PCR test for the colonies from these 4 mediums.
From those incubated plates, 4 colonies (2 from FNR+plasmid PSB1C3, 2 from LacZ and AmilCP) were selected in parallel for further test.
Used 4 primers: VF2, VR, PFNR_up, PFNR_down for verification. They were designed to cut 4 special sites for creating 3 different regions on plasmid chain: VF2/VR, VF2/PFNR_down, PFNR_up/VR. The PCR products had been put on electrophoresis gel for the verification

constructing