Team:TU-Eindhoven/Notebook
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In this section you can find a small description of all the work we have done so far organized by the category it belongs to.
Feb-Apr
- Background research
- Selection of topic
- Further research
- Selection of project
- Further research
May
General Activities
- Regular team meeting
- Looking for sponsors
- Month planning
Wetlab
- Researching about the promoters
- Researching about the possible proteins for CEST imaging
Drylab
- Defining what needs to be modeled and what type of model is the most appropriate for it
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Researching and defining the killing mechanism
Drylab
- Starting the Molecular Dynamics model for the proteins selection.
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Defining the needed materials
- Defining the needed equipment
Drylab
- Continuing the Molecular Dynamics model for the proteins selection.
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Locating, booking and learning how to use the necessary equipment
Drylab
- Analyzing the results from the Molecular Dynamics model for the proteins selection and identifying the most useful proteins for CEST imaging
- Researching and defining the needed parameters for the analysis of the decoy sites through a stochastic simulations model
Human Practices
- Selecting a main project
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Locating, booking and learning how to use the necessary equipment
Drylab
- Starting the stochastic simulations for the analysis of the decoy sites
Human Practices
- Selecting a main project
June
General Activities
- Regular team meeting
- Looking for sponsors
- Collaborating with Purdue iGEM team
- Planning month activities
Wetlab
- General construct design
Drylab
- Continuing the stochastic simulations for the analysis of the decoy sites
Human Practices
- Defining the structure of the fact checker project
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Protein design
Drylab
- Continuing the stochastic simulations for the analysis of the decoy sites
Human Practices
- Redacting the preliminary description and survey for collaborators of the fact checker project
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Final design of the constructs
Drylab
- Continuing the stochastic simulations for the analysis of the decoy sites
Human Practices
- Revising the preliminary description and survey for collaborators of the fact checker project
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Ordering the constructs
- Working on the protocols
Drylab
- Analyzing the results from the stochastic simulations for the analysis of the decoy sites
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
July
General Activities
- Regular team meeting
- Looking for sponsors
- Planning month activities
Wetlab
- Finalizing the protocols
- Planning lab schedule
Drylab
- Starting the ODE model for the analysis of the FNR promoter
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
General Activities
- Regular team meeting
- Looking for sponsors
- Collaborating with Purdue iGEM team
Drylab
- Continuing the ODE model for the analysis of the FNR promoter
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
General Activities
- Regular team meetings
- Looking for sponsors
- Collaborating with Purdue iGEM team
Wetlab
- Creating agar plates
- Transforming DNA
- Plating bacteria
- Making LB medium
- Creating small cultures
- DNA retention from cultures
- Nano-drop tests
Drylab
- Analyzing the results from the ODE model for the analysis of the FNR promoter
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
- Sending the survey to collaborators
General Activities
- Regular team meetings
- Looking for sponsors
Wetlab
- Creating agar plates
- Transforming DNA
- Plating bacteria
- Making LB medium
- Creating small cultures
- DNA retention from cultures
- Nano-drop tests
Drylab
- Merging the stochastic simulations model for the decoy sites with the ODE model for the FNR promoter
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
August
General Activities
- Regular team meetings
- Looking for sponsors
- Working on outreach ideas
Wetlab
- Creating the Cultures
- Retaining the DNA
- Nanodrop Test
Drylab
- Merging the stochastic simulations model for the decoy sites with the ODE model for the FNR promoter
- Working on the pharmacokynetics model
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
- Working on the fact checker web platform
- Redacting the first facts to be added on our fact checker platform
General Activities
- Regular team meetings
- Looking for sponsors
- Working on outreach ideas
Wetlab
- Creating the Cultures
- Retaining the DNA
- Nanodrop Test
Drylab
- Improving the merged model
- Making an ODE model of the decoy sites
- Working on the pharmacokynetics model
Human Practices
- Working on the fact checker web platform
- Preparing the instructions and example files for collaborating teams
General Activities
- Regular team meetings
- Looking for sponsors
- Working on outreach ideas
- Sharing our project with colleagues and faculty members
Wetlab
- Digestion
- Construct Digestion
- Vector Digestion
- PCR Purification for the Constructs
- PCR Puricfication for the Vectors
- Nanodrop Results
- Continuing with the Purification
- Vector Purification
- Construct Purification
- Ligation
- Preparing Agar Plates
- Transformation
- Plating
- Colony Picking and PCR
- Culturing the Ligation Products
Drylab
- Sensitivity analysis of the merged model
- Estimating promotor rates
- Working on the pharmacokynetics model
Human Practices
- Working on the fact checker web platform
General Activities
- Regular team meetings
- Looking for sponsors
- Working on outreach ideas
Wetlab
- Colony Picking and PCR
- Small Cultures and Preparation for Protein Expression
- Miniprepping New Constructs
- Sequencing
- Trnasformation and Plating
- Expressing Samples
- Protein Purification
- Determining Protein Concentration
- SDS Analysis
- Performing the Protein Expression in an Anaerobic Environment
- MRI Experiments
Drylab
- Working on the pharmacokynetics model
Human Practices
- Fact checker platform is up and running
- ...
September
- ...
- ...
- ...
- ...