Team:Paris Saclay/Notebook/July/15

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Contents

1 Notebook : July 10
- 1.1 Lab work
  - 1.1.1 A - Aerobic/Anaerobic regulation system
    - 1.1.1.1 Objective : obtaining Bba_K1155003, Bba_K1155007
    - 1.1.1.2 1 - Transformation of Bba_I732019 and Bba_B0010 in DH5α
  - 1.1.2 B - PCB sensor system
    - 1.1.2.1 Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2
    - 1.1.2.2 1 - Digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments

Notebook : July 10

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155003, Bba_K1155007

1 - Transformation of Bba_I732019 and Bba_B0010 in DH5α

B - PCB sensor system

Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2

1 - Digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments

Abdou

Used quantities :

EcoRI/PstI :
- DNA : 8µL
- Buffer Orange : 1.6µL
- EcoRI : 1µL
- PstI : 1µL
- H2O : 4.4µL

SacII :
- DNA : 8µL
- Buffer Blue : 1.6µL
- SacII : 1µL
- H2O : 5.4µL

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