Notebook : August 19

Lab work

A - Aerobic/Anaerobic regulation system

Objective : to obtain ...

1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI and PstI

Nadia

Protocol : Gel purification

Nanodrop :

• RBS-LacZ-Term : 59.6ng/µL
• RBS-AmilCP-Term : 21.3ng/µL

Concentrations are very low so we try to precipitate the DNA again.  

2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term

Nadia, XiaoJing

Protocol : EtOH precipitation

We used 20µL of DNA.

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : to obtain ...

1 - Electrophoresis gel of BphR2 produce by PCR

Damir

Well 1 : 6µL DNA Ladder Well 2 : 5µL DNA + 1µl of 6X loading dye Gel : 1%

We obtain the BphR2 by PCR.

2 - Gel purification of BphR2

Damir

Protocol : Gel purification

Nanodrop :

• BphR2 : 57.2ng/µL

CONCLUSION

Meeting with Evry