Team:Paris Saclay/Notebook/August/20

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Notebook : August 20

Lab work

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : obtaining FNR and BphR2 proteins

1 - PCR of BphR2 Part I

Damir

Used quantities :

PCR Program :

2 - Electrophoresis of PCR products : BphR2 Part I

Nadia

[[]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 40µL BphR2 PartI+8µl of 6X loading dye
  • Gel : 0.8%

We obtain a frangment at the right size. We can purify it.

2 - Gel purification of PCR products : BphR2 Part I

Damir, Nadia

Protocol : Gel purification

Nanodrop :

  • BphR2 Part I: .........

CONCLUSION ON A TOUS PERDU D'OU ON RECOMMENCE ENSUITE ??????


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