Team:Paris Saclay/Notebook/July/31

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Notebook : July 31

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K155006

1 -Ligation of NarK, NarG, NirB in PSB1C3

XiaoJing

Used quantities :

  • Buffer : 2µL
  • NarK, NarG, NirB : 1µL
  • PSB1C3 : 2µL
  • Ligase : 1µL
  • H2O : 14µL

2 -Electrophoresis to check the ligation of NarK, NarG, NirB in PSB1C3

XiaoJing

[[]]
  • Well 1 : 6µL of DNA Ladder
  • Well 2 : 5µl of NirB+1µl 6X loading dye
  • Well 3 : 5µl of NirB+1µl 6X loading dye
  • Well 4 : 5µl of NarG+1µl 6X loading dye
  • Well 5 : 5µl of NarG+1µl 6X loading dye
  • Well 6 : 5µl of NarK+1µl 6X loading dye
  • Well 7 : 5µl of NarK+1µl 6X loading dye
  • Gel : 0.8%

Expected sizes :

  • NarK : ...
  • Nar G : ...
  • Nir B : ...

We obtain fragments at the right size. We will transform them in DH5α.

3 -Tranformation of Bba_K1155004, Bba_K1155005, Bba_K1155006

Anaïs

Protocol : Bacterial transformation

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