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• iGEM 2013

Phage Purification May - June Notebook: May 27 - June 9 Daily Log

Overview March-April May-June July-August September-October

6/10/13 - Prepared RNase for future use. - Prepared W3110 and BL21 cultures. 6/10/13 - Prepared RNase for future use. - Prepared W3110 and BL21 cultures. 6/3/13 - Ran the CsCl purification step. 6.3 CsCl Gradient Phage Purification - Next class we will attempt extracting the phage from the gradient. 6/5/13 - Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle. Only extracted T4 because the T7 gradient was destroyed in the refrigerator. Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed. Placed these in the refrigerator. - Prepared stocks of bacteria for future use. 6.5 Bacteria Cultures 6/7/13 Made 1 L of 10 X phage suspension buffer. 6.7 Phage Suspension Buffer Preparation