Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog

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Phage Purification May - June Notebook: June 10 - June 23 Daily Log

6/3/13

- Ran the CsCl purification step.

- Next class we will attempt extracting the phage from the gradient.

6/5/13

- Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle.

Only extracted T4 because the T7 gradient was destroyed in the refrigerator.

Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.

Placed these in the refrigerator.

- Prepared stocks of bacteria for future use.

6/7/13

Made 1 L of 10 X phage suspension buffer.

6/10/13

- Prepared RNase A for future use.

- Prepared T4 and T7 cultures.

6/12/13

- Began first step of purification process using phage from 6.10 Phage Preparation.