Team:BYU Provo/Notebook/Cholera - Enzymes/July-August

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Cholera - Enzyme Notebook July - August
Cholera Detection March-April May-June July-August September	July 1 - July 14 We continued to have issues with washing our biofilm during the biofilm assay without losing significant portions of the biofilm. We tried several different methods to try to find the best method for treating V. cholerae biofilm specifically. After several different approaches, we found that the most consistent way to treat the biofilm during the assay is to pellet and resuspend the biofilm with each wash, allowing us to wash our sample without losing the biofilm itself. Daily log
	July 15 - July 31 We focused these two weeks on preparing our DspB and Savinase enzymes for protein purification so we could test them in our Biofilm Assay. We ran PCRs, restriction digests, ligations, and transformations on them. Daily log
	August 1 - August 14 We checked the transformation products from our earlier work with colony PCR, but the transformations were unsuccessful. We worked this period on trying to get our ligations and transformations to work. Daily log
	August 15 - August 31 We continued working on getting DspB and Savinase ready for protein purification. We were having so many problems with Savinase that we went back and prepared new template DNA from Bacillus subtilis to use for our Savinase enzyme. Daily log