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Contents 1 Team CRISPR 1.1 July 3 2 July 10th 3 September 22nd

Team CRISPR

July 3

List of BioBricks that are/will be on the way:

Cas9:

• Cas9 - Joe and Anna
• pTet -
• const. promoter + Cas9 - Joe
• promoter + Cas9 + Leader + R + spacer + R (on Kan)
• L + R + spacer + R

Target:

• tracerRNA
• repeat + spacer + repeat - Vanins*2 + underlings
• leader - Ray, Fisal, Dan

Decoy:

• PAM + spacer (on Amp)

July 10th

Experimenter:Anna Müller

"Aim:" PCR the cas9 gene from Streptococcus thermophilus cells.

Results: Unsuccessful no bands were seen on the agarose gel.

September 22nd

Experimenter: Anna Müller

Aim: Digest T4, T7 spacer sequence with PAM site(decoy construct) with EcoRI and SpeI to later on ligate into plasmid with ampicillin resistance.

Results: will follow.

Aim: Digest PsBIA3 plasmid with ampicillin resistance with EcoRI and SpeI to construct the decoy plasmides.

Results: will follow.