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Team:British Columbia/Notebook/CRISPR
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Team CRISPR
July 3
List of BioBricks that are/will be on the way:
Cas9:
- Cas9 - Joe and Anna
- pTet -
- const. promoter + Cas9 - Joe
- promoter + Cas9 + Leader + R + spacer + R (on Kan)
- L + R + spacer + R
Target:
- tracerRNA
- repeat + spacer + repeat - Vanins*2 + underlings
- leader - Ray, Fisal, Dan
Decoy:
- PAM + spacer (on Amp)
July 10th
Experimenter: Anna Müller
Aim: PCR the cas9 gene from Streptococcus thermophilus cells.
Results: Unsuccessful no bands were seen on the agarose gel.
September 22nd
Experimenter: Anna Müller
Aim: Digest T4, T7 spacer sequence with PAM site(decoy construct) with EcoRI and SpeI to later on ligate into plasmid with ampicillin resistance.
Results: will follow.
Aim: Digest PsBIA3 plasmid with ampicillin resistance with EcoRI and SpeI to construct the decoy plasmides.
Results: will follow.
