Gel Extraction

Performed with AxyPrep 96-well DNA Gel Extraction Kit (type: AP-96-GX) Protocol
1. Excise gel slice containing DNA fragment of interest.
2. Add 3×sample volume of Buffer DE-A.
Incubate at 75° C for 15-20 min or until gel melts completely. Add 0.5 × Buffer DE-A volume of Buffer DE-B.

3. Binding sample DNA(Centrifuge at 5,000 - 6,000×g for 5 min).

4. Add 800 μl of Buffer W2 (5,000 - 6,000×g for 1 min).
Repeat wash with Buffer W2.
Centrifuge empty plate for 5 min at 5,000 - 6,000×g to remove residue W2.

5. Elute with 50 μl of Eluent or Deionized Water.(5,000 - 6,000×g, 5min).