Team:NYMU-Taipei/Modeling/MainParts

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National Yang Ming University


Function of the parts:

  1. circuit regulators:
  • LacIregulatedpromoter(pLac) : when LacI exists, it will bind to LacIregulatedpromoter(pLac) and represses the promoter.
  • pLux/cIhybridpromoter(plux/cI) : when luxR/AHL exists, it will open pLux/cIhybridpromoter(plux/cI), while cI will repress the promoter. What’s more, plux/cI is cI-dominant, which means when cI exists, the hybrid promoter will be repressed whether luxR/AHL exists or not.
  1. Circuit regulation:
  • Circuit condition without Nosema:
NYMU-Taipei Circuit condition wo nosema.jpg

Without NosemaCeranae, the first circuit will not open, and thus, no LacI will be produced. Since there is no LacI binding to pLac, pLac will not be repressed, which means cI will be produced. After that, cIwill bind to pLux/cI-hybridpromoter, leading the third circuit to be off.

  • Circuit condition with Nosema:
NYMU-Taipei Circuit condition w nosema.jpg


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