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Team:TU-Delft/Timer
From 2013.igem.org
Timer
A timer was added to the total circuit in order to control the peptide production. The timer has two possible states: can be either on or off. In particular,timer is inactivated(off) when there is no S.aureus detected and activated(on) in the opposite case.Two are main parts of the timer circuit that control its function: the pTet promoter and the pcI promoter(Figure 1).
As long as there is no S.aureus, the pTet promoter is on. If pTet promoter is on, then there is no CI produced that can lead to the repression of pcI promoter and consequently to the ulp activation which cleaves an inactivating tag leading to peptide production.
In case that S.aureus is detected, then the pTet promoter is repressed. The repression of pTet results in the activation of cI which represses pcI. In that way, ulp is activated, the inactivated tag is cleaved and the peptide is start produced.
