Team:Paris Saclay/Protocols

From 2013.igem.org

Revision as of 14:13, 28 September 2013 by Nad' (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Labwork Overview Biobricks Protocols Safety

Protocols

Transformation of bacteria super competent cells

Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue

Preparation of bacteria super competent cells

PCR for the genomic DNA of bacteria

Plasmidic DNA Mini and Maxi preparation

Electro-elution

Gibson Assembly

Electrophoresis on gel

Ethanol Precipitation

Digestion and Ligation

[http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]

Protocols written by Sheng (the 4 first), Nadia (Transduction) and Abdou

Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Protocols"