iGEM Evry 2013 achievements

Here we detail how we fulfilled each of the iGEM medal requirements.

Gold Medal (requires any one of the following goals)

• (Improve the function of an existing BioBrick Part or Device) => LacO RFP
• Help any registered iGEM team
• Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing.

Silver Medal (requires each of the four following goals)

• We experimentally validate the aceB-sfGFP biobrick that we submitted to the iGEM Parts registry using a TECAN and microfluidics.
• We document the characterization of our aceB-sfGFP and other Biobricks in "Main Page" section of the Parts Registry.
• We submitted the following Biobrick parts to the iGEM Parts Registry.
  • BBa_K1163102: AceB-sfGFP characterization
  • BBa_K1163103: AceB-LacI characterization
  • BBa_K1163105: FepA-sfGFP characterization
  • BBa_K1163106: FepA-LacI characterization
  • BBa_K1163108: Fes-sfGFP characterization
  • BBa_K1163109: Fes-LacI characterization
  • BBa_K1163111: YncE-sfGFP characterization
  • BBa_K1163112: YncE-LacI characterization
• We describe how our project has implications for safety and ethics

Bronze Medal (requires each of the five following goals)

• We registered our team for iGEM 2013 and are attending the European Regional Jamboree
• We completed and submitted the Judging form
• We made a comprehensive and accessible iGEM Evry 2013 Wiki to convey our educational and scientific advances
• We will present a poster and a talk at the iGEM European Jamboree in Lyon, France.
• We document xx BioBricks and submit 8 news BioBricks used in our project:
  • BBa_K1163102: AceB-sfGFP
  • BBa_K1163103: AceB-LacI
  • BBa_K1163105: FepA-sfGFP
  • BBa_K1163106: FepA-LacI
  • BBa_K1163108: Fes-sfGFP
  • BBa_K1163109: Fes-LacI
  • BBa_K1163111: YncE-sfGFP
  • BBa_K1163112: YncE-LacI

And more precisely ?!

Biology

• We design and create 4 Iron sensing systems, that could also works with an invertor !
• We design an Iron chelating system and isolate genes of the enterobactins biosynthesis pathway !
• We use TECAN and microfluidic to analyse our systems and show that they work !
• We created a pill adapted to our project and that fulfilled basic requirements of the European Pharmacopeia !

Model

Human Practices

• We make a series of interviews and discussion with experts on iron overload diseases.
• We make reflexion about the safety of potential consequences of our bacteria, including a consequence tree.
• We had discussion with hemochromatosis patient and held a seminar on iron overload and on our project on 18 September 2013.
  Scientists working on iron overload, patients, and students attended to it !
• We make a survey about hemochromatosis and about our potential treatment.
  We received and analyzed more than 250 answers from patients !
• We submitted an extensive report of our conclusions about synthetic biology , which is the fruit of our long reflection we conducted over the summer.