iGEM Evry 2013 achievements

Here we detail how we fulfilled each of the iGEM medal requirements.

Gold Medal (requires any one of the following goals)

• (Improve the function of an existing BioBrick Part or Device) => LacO RFP
• Help any registered iGEM team
• As our project involves treating a human genetic disorder using genetically-engineered bacteria, it has numerous implications for human health, safety and security, and ethics. We explored these issues as follows:
  1. We discussed the medical implications of this treatment in a series of interviews and discussion with renowned experts on iron overload diseases.
  2. We carefully considered the safety issues and potential consequences of our bacteria, which are illustrated in this consequence tree.

Silver Medal (requires each of the four following goals)

• We experimentally validate the aceB-sfGFP biobrick that we submitted to the iGEM Parts registry using a TECAN and microfluidics.
• We document the characterization of our aceB-sfGFP and other Biobricks in "Main Page" section of the Parts Registry.
• We submitted the following Biobrick parts to the iGEM Parts Registry.
  • BBa_K1163102: AceB-sfGFP characterization
  • BBa_K1163103: AceB-LacI characterization
  • BBa_K1163105: FepA-sfGFP characterization
  • BBa_K1163106: FepA-LacI characterization
  • BBa_K1163108: Fes-sfGFP characterization
  • BBa_K1163109: Fes-LacI characterization
  • BBa_K1163111: YncE-sfGFP characterization
  • BBa_K1163112: YncE-LacI characterization
• We describe how our project has implications for safety and ethics

Bronze Medal (requires each of the five following goals)

• We registered our team for iGEM 2013 and are attending the European Regional Jamboree
• We completed and submitted the Judging form
• We made a comprehensive and accessible iGEM Evry 2013 Wiki to convey our educational and scientific advances
• We will present a poster and a talk at the iGEM European Jamboree in Lyon, France.
• We document xx BioBricks and submit 8 news BioBricks used in our project:
  • BBa_K1163102: AceB-sfGFP
  • BBa_K1163103: AceB-LacI
  • BBa_K1163105: FepA-sfGFP
  • BBa_K1163106: FepA-LacI
  • BBa_K1163108: Fes-sfGFP
  • BBa_K1163109: Fes-LacI
  • BBa_K1163111: YncE-sfGFP
  • BBa_K1163112: YncE-LacI

And more precisely ?!

Biology

• We design and create 4 Iron sensing systems, that could also works with an invertor !
• We design an Iron chelating system and isolate genes of the enterobactins biosynthesis pathway !
• We use TECAN and microfluidic to analyse our systems and show that they work !
• We created a pill adapted to our project and that fulfilled basic requirements of the European Pharmacopeia !

Model

Human Practices

• We had discussion with hemochromatosis patient and held a seminar on iron overload and on our project on 18 September 2013.
  Scientists working on iron overload, patients, and students attended to it !
• We make a survey about hemochromatosis and about our potential treatment.
  We received and analyzed more than 250 answers from patients !
• We submitted an extensive report of our conclusions about synthetic biology , which is the fruit of our long reflection we conducted over the summer.