Biological Modules: Carry

For the carry we thought of a molecule that could transmit a message from one colony to the other.
The communication between cells in Quorum Sensing is a good illustration of the diffusion of a signal between unicellular organisms. We resorted to the system present in Vibrio fischeri using N-Acyl homoserine lactone (AHL).

General principle
AHL is the product of the LuxI gene. This one was placed under the control of promoters belonging to the two AND gates. So, AHL will be produced when these gates will be activated and transmitted to the following colony by diffusion into LBagar and Quorum Sensing. It becomes a third input signal (in addition of blue/red light) to take in consideration for the calculation of the next bit.

Carry module characterization
AHL diffusion into agar medium was characterized thanks to the Chromobacterium violaceum strain. A purple coloration is visible when C. violaceum is in contact with AHL.